Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43910843" target="_blank" >RIV/62156489:43210/17:43910843 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/17:00096688 RIV/00216305:26620/17:PU123000

Result on the web

<a href="http://dx.doi.org/10.1016/j.snb.2016.11.160" target="_blank" >http://dx.doi.org/10.1016/j.snb.2016.11.160</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2016.11.160" target="_blank" >10.1016/j.snb.2016.11.160</a>

Result language

angličtina

Original language name

Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution

Original language description

Oxidation stress induced by nanoparticles is considered to be the main mechanism of their toxicity. An evaluation of oxidative damage to DNA, one of the mostly endangered molecules, belongs to general approaches used in nanomaterials cytotoxicology. However, for spherical, biochemical and other reasons, they should be differences in nanoparticles behaviour using tests based on cells and pure nucleic acid substances. The goal of the work is to detect structural damage to isolated salmon sperm double stranded DNA at its exposure to UV-C radiation (254 nm) in the presence of thiol-capped CdS quantum dots of various size and fluorescence intensity. An electrochemical DNA-based biosensor composed of a glassy carbon electrode with a layered structure of chemical modifiers dsDNA and CdS QDs was prepared and used for the voltammetric and impedimetric evaluation of a DNA portion survived after the irradiation. The cyclic voltammetric response of the redox indicator system hexacyanoferrate(III/II) and square wave voltammetric response of the guanine and adenine moieties were measured as analytical signals. The results were verified by experiments with irradiation of DNA in the solution phase followed by voltammetric measurement of immobilized survived portion of DNA (biosensing) and by conventional agarose gel electrophoresis. Experiments showed significant effect of the fluorescent active quantum dots on the degree of DNA degradation.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors and Actuators B. Chemical

ISSN

0925-4005

e-ISSN

—

Volume of the periodical

243

Issue of the periodical within the volume

May

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

435-444

UT code for WoS article

000395969100055

EID of the result in the Scopus database

2-s2.0-85007179510