COL1A1 (type I collagen) gene expression in wounded skin of rats was not significantly influenced by docosahexaenoic (DHA) and eicosapentaenoic (EPA) acid enrichment of the diet

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914694" target="_blank" >RIV/62156489:43210/18:43914694 - isvavai.cz</a>

Result on the web

<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

COL1A1 (type I collagen) gene expression in wounded skin of rats was not significantly influenced by docosahexaenoic (DHA) and eicosapentaenoic (EPA) acid enrichment of the diet

Original language description

The main aim of this study was to compare expression of collagen I gene (COL1A1) in the healing skin of rats fed the diets enriched with docosahexaenoic (DHA) and eicosapentaenoic (EPA) fatty acid. The total of fifty Wistar Albino rats were divided into five groups (ten animals in each group). The animals were fed the basic mixture with addition of Schizochytrium microalga extract (D), palm oil (P), fish oil (R), and safflower oil (S), respectively. Group K served as a control fed the basic feed mixture alone. Animals were fattened eight weeks by respective diets and then excisions on their back were made by puncher in anesthesia. RNA was isolated from the samples of the healing tissue ten days post-excision and expression of the COL1A1 and β-actin (reference gene) was measured by the realtime PCR. COL1A1 gene expression in the D, P, R and S samples, did not differ significantly (p&gt;0.05) from the control (K), therefore the hypothesis that EPA/DHA increase COL1A1 expression was not proved.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

MendelNet 2018: Proceedings of International PhD Students Conference

ISBN

978-80-7509-597-8

ISSN

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e-ISSN

neuvedeno

Number of pages

3

Pages from-to

399-401

Publisher name

Mendelova univerzita v Brně

Place of publication

Brno

Event location

Brno

Event date

Nov 7, 2018

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

000462205300076