SSR loci survey of technical hemp cultivars: The optimization of a cost-effective analyses to study genetic variability
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43918061" target="_blank" >RIV/62156489:43210/20:43918061 - isvavai.cz</a>
Alternative codes found
RIV/26784246:_____/20:N0000035
Result on the web
<a href="https://doi.org/10.1016/j.plantsci.2020.110551" target="_blank" >https://doi.org/10.1016/j.plantsci.2020.110551</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.plantsci.2020.110551" target="_blank" >10.1016/j.plantsci.2020.110551</a>
Alternative languages
Result language
angličtina
Original language name
SSR loci survey of technical hemp cultivars: The optimization of a cost-effective analyses to study genetic variability
Original language description
Our study aimed to optimize a selection of a suitable combination of SSRs (Simple Sequence Repeats) for determination of technical Cannabis cultivars and genetic variability level. We used sequences of 23 published SSR families (107 alleles) and amplified them in 28 cultivars. One of the alleles possesses no selective information (SSR family CAN1660) due to its presence in every single tested cultivar. We excluded it, together with another 11 least informative alleles. After data filtration, we used 96 alleles to do recursive sub-sampling of random alleles' sets. We found a minimal set of 8 alleles (in three different combinations) to distinguish 28 analyzed cultivars from each other. Our results contribute to saving resources and to reduce the performance time of the molecular analysis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Science
ISSN
0168-9452
e-ISSN
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Volume of the periodical
298
Issue of the periodical within the volume
September
Country of publishing house
IE - IRELAND
Number of pages
7
Pages from-to
110551
UT code for WoS article
000557874400008
EID of the result in the Scopus database
2-s2.0-85087302533