One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43918131" target="_blank" >RIV/62156489:43210/20:43918131 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/20:00118337 RIV/00216305:26620/20:PU137914

Result on the web

<a href="https://doi.org/10.1016/j.jcis.2020.06.125" target="_blank" >https://doi.org/10.1016/j.jcis.2020.06.125</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jcis.2020.06.125" target="_blank" >10.1016/j.jcis.2020.06.125</a>

Result language

angličtina

Original language name

One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity

Original language description

In the present study, the thermal decomposition of citric acid in the presence of biogenic amine was used to synthesize four different functionalized carbon quantum dots (CQDs), namely, histamine-(HCQDs), putrescine-(PCQDs), cadaverine-(CCQDs) and spermine-(SCQDs). The thermal decomposition of the precursors resulted in a decrease in stability and the formation of surface amides via a cross-linking process between the carboxyl and amine groups. The deposition of biogenic amines was confirmed by a structural characterization of the synthesized CQDs. The resulting CQDs, with a net zero charge, exhibited excellent stability in environments with different pH values. Through a set of different cytotoxicity tests, the absence of gene mutations, apoptosis, necrosis or disruption in cell membranes revealed the high biocompatibility of the CQDs. The antimicrobial activity of the synthesized CQDs was investigated against different bacterial species (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumonia). We determined the growth kinetics, production of reactive oxygen species (ROS), cell viability and changes in membrane integrity by scanning electron microscopy (SEM). The minimal inhibitory concentrations (MICs) for S. aureus ranged from 3.4 to 6.9 µg/mL. Regarding E. coli and K. pneumonia, all CQD formulations reduced growth, and the MICs were determined for CCQDs and HCQDs (6.9-19.4 µg/mL). The antibacterial activity mechanism was attributed to the oxidative stress generated after CQD treatment, which resulted in the destabilization of the bacterial membrane. The bacterial permeability to propidium iodide indicated a change in membrane integrity, and the effect of CQDs on the morphology of the bacterial cells was evidenced by SEM.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10403 - Physical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Colloid and Interface Science

ISSN

0021-9797

e-ISSN

—

Volume of the periodical

580

Issue of the periodical within the volume

15 November

Country of publishing house

US - UNITED STATES

Number of pages

19

Pages from-to

30-48

UT code for WoS article

000581780400004

EID of the result in the Scopus database

2-s2.0-85087861150