Optimization of assay for total protein in the haemolymph of the honeybee (Apis mellifera L.)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919608" target="_blank" >RIV/62156489:43210/20:43919608 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43410/20:43919608
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Optimization of assay for total protein in the haemolymph of the honeybee (Apis mellifera L.)
Original language description
Protein levels in honeybee is a credible marker of a bee physiological status. Bradford's method is suitable for quantification of the total protein in the haemolymph. Two types of diluents, different diluting ratios and impact of storage were compared. Average decrease of the total protein content after one week storage of samples was lower in samples diluted in the phosphate buffer pH 7 in comparison with ones diluted with MilliQ water. The haemolymph samples dissolved in the phosphate buffer showed significantly higher yield and lower variability of the total protein content compared to the variability obtained by dissolving the haemolymph in MilliQ water. These results indicate that 200x dilution in the phosphate buffer (pH = 7) seems to be optimized for determination of the total protein in the bee haemolymph.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2020: Proceedings of International PhD Students Conference
ISBN
978-80-7509-765-1
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
530-535
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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