Feasible delivery system based on poly(lactictide-co-glycolide) nanoparticles loaded with antimicrobial mupirocin for possible wound healing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F23%3A43923991" target="_blank" >RIV/62156489:43210/23:43923991 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.2754/avb202392030279" target="_blank" >https://doi.org/10.2754/avb202392030279</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb202392030279" target="_blank" >10.2754/avb202392030279</a>
Alternative languages
Result language
angličtina
Original language name
Feasible delivery system based on poly(lactictide-co-glycolide) nanoparticles loaded with antimicrobial mupirocin for possible wound healing
Original language description
The objective of the study was to assess cytotoxicity (based on the dimethylthiazol- diphenyltetrazolium bromide cell viability assay) and antimicrobial effects of poly(lactictide-co-glycolide) nanoparticles with entrapped mupirocin (PLGA/MUP NPs) on Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) strains using a disk-diffusion method, cryo-scanning electron microscopy (cryo-SEM) and fluorescence microscopy. Based on the evaluation of the growth curve, PLGA/MUP NPs inhibited growth of the both tested strains already at a concentration of 0.29 µg/ml, and their inhibitory effect at concentrations from 0.29 to 1.17 µg/ml was comparable with free MUP using the disk-diffusion method. PLGA/MUP NPs also tended to increase the abundance of the dead cells of MRSA, but not of S. aureus, in comparison with free MUP when evaluated by fluorescence microscopy. Further, cryo-SEM evaluation demonstrated an antibacterial-inhibitory effect of PLGA/MUP NPs on S. aureus in a dose-dependent manner. On the other hand, PLGA/MUP NPs cytotoxic activity tended to be substantially lower in comparison with both free MUP and empty PLGA NPs. It can be concluded that the excellent biocompatibility and satisfactory antibacterial effects of PLGA/MUP NPs constitute a suitable alternative as far as cutaneous wound healing is concerned.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EF16_025%2F0007314" target="_blank" >EF16_025/0007314: Multidisciplinary research to increase application potential of nanomaterials in agricultural practice</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Veterinaria Brno
ISSN
0001-7213
e-ISSN
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Volume of the periodical
92
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
9
Pages from-to
279-287
UT code for WoS article
001153359000008
EID of the result in the Scopus database
2-s2.0-85168802217