Mitochondrial bioenergetics linked to the manifestation of programmed cell death during somatic embryogenesis of Abies alba
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F09%3A00146508" target="_blank" >RIV/62156489:43410/09:00146508 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Mitochondrial bioenergetics linked to the manifestation of programmed cell death during somatic embryogenesis of Abies alba
Original language description
The present work reports changes in bioenergetic parameters and mitochondrial activities during the manifestation of two events of programmed cell death (PCD), linked to Abies alba somatic embryogenesis. PCD, evidenced by in situ nuclear DNA fragmentation (TUNEL assay), DNA laddering and cytochrome c release, was decreased in maturing embryogenic tissue with respect to the proliferation stage. In addition, the major cellular energetic metabolites (ATP, NAD(P)H and glucose-6- phosphate) were highered during maturation. The main mitochondrial activities changed during two developmental stages. Mitochondria, isolated from maturing, with respect to proliferating cell masses, showed an increased activity of the alternative oxidase, external NADH dehydrogenase and fatty-acid mediated uncoupling. Conversely, a significant decrease of the mitochondrial KATP ? channel activity was observed. These results suggest a correlation between mitochondrial activities and the manifestation of PCD duri
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Planta
ISSN
0032-0935
e-ISSN
—
Volume of the periodical
—
Issue of the periodical within the volume
231
Country of publishing house
DE - GERMANY
Number of pages
15
Pages from-to
—
UT code for WoS article
—
EID of the result in the Scopus database
—