Assessing the potential of lignin nanoparticles as drug carrier: Synthesis, cytotoxicity and genotoxicity studies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F20%3A43917657" target="_blank" >RIV/62156489:43410/20:43917657 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.ijbiomac.2020.02.311" target="_blank" >https://doi.org/10.1016/j.ijbiomac.2020.02.311</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2020.02.311" target="_blank" >10.1016/j.ijbiomac.2020.02.311</a>

Result language

angličtina

Original language name

Assessing the potential of lignin nanoparticles as drug carrier: Synthesis, cytotoxicity and genotoxicity studies

Original language description

Lignin nanoparticles synthesis is among recent developments in lignin valorization especially for biomedical applications. In this study, a new technique where complete self-assembling of lignin was ensured by simultaneous solvent displacement and flash pH change was used to optimize particle size of blank lignin nanoparticles (BLNPs) for suitability in cell uptake along with maximized yield. To establish BLNPs as drug carrier, safety studies including hemocompatibility, cytotoxicity and elaborate genotoxicity studies on Drosophila melanogaster as a model organism were done. Finally, irinotecan loaded lignin nanoparticles (DLNPs) were synthesized to establish their drug carrying potential and thorough in vitro characterization was performed. BLNPs with controllable size (⁓152 nm), low polydispersity (&lt;0.2), maximized yield (&gt;65%), negative surface charge (MINUS SIGN 22 to MINUS SIGN 23 mV), spherical shape and smooth surface were obtained with acceptable %hemolysis (&lt;2%). In vitro cytotoxicity studies revealed that BLNPs were significantly toxic (74.38 +- 4.74%) in human breast adenocarcinoma (MCF-7), slightly toxic (38.8 +- 4.70%) in human alveolar epithelial adenocarcinoma (A-549) and insignificantly toxic (15.89 +- 2.84%) to human embryonic kidney (HEK-293) cells. BLNPs showed concentration dependent early neuronal defects in Drosophila, but nuclei fragmentation and gut cell damage were absent. Sustained release DLNPs with high drug loading reduced the IC50 value of irinotecan by almost 3 folds.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

—

Volume of the periodical

152

Issue of the periodical within the volume

1 June

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

17

Pages from-to

786-802

UT code for WoS article

000530068000078

EID of the result in the Scopus database

2-s2.0-85081321551