Self-monitoring and self-delivery of self-assembled fluorescent nanoparticles in cancer therapy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F21%3A63529118" target="_blank" >RIV/70883521:28610/21:63529118 - isvavai.cz</a>

Result on the web

<a href="https://www.dovepress.com/self-monitoring-and-self-delivery-of-self-assembled-fluorescent-nanopa-peer-reviewed-article-IJN" target="_blank" >https://www.dovepress.com/self-monitoring-and-self-delivery-of-self-assembled-fluorescent-nanopa-peer-reviewed-article-IJN</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2147/IJN.S294279" target="_blank" >10.2147/IJN.S294279</a>

Result language

angličtina

Original language name

Self-monitoring and self-delivery of self-assembled fluorescent nanoparticles in cancer therapy

Original language description

Purpose: Due to the shortcomings of nanocarriers, the development of carrier-free nanodelivery systems has attracted more and more attention in cancer treatment. However, there are few studies on carrier-free nanosystems that can simultaneously achieve monitoring functions. Here a multifunctional carrier-free nanosystem loaded with curcumin and irinotecan hydrochloride was established for the treatment and monitoring of gastric cancer. Methods: In this study, an irinotecan hydrochloride-curcumin nanosystem in the early stage (the system is named SICN) was prepared. Based on the fluorescence of curcumin, flow cytometry, laser confocal microscopy, and zebrafish fluorescence imaging were used to study the monitoring function of SICN in vivo and in vitro. In addition, HGC-27 human gastric cancer cells were used to study SICN cytotoxicity. Results: Flow cytometry and zebrafish fluorescence imaging monitoring results showed that the uptake of SICN was significantly higher than free curcumin, and the excretion rate was lower. SICN had higher accumulation and retention in cells and zebrafish. Laser confocal microscopy monitoring results showed that SICN was internalized into HGC-27 cells through multiple pathways, including macropinocytosis, caveolin, and clathrin-mediated and clathrin-independent endocytosis, and distributed intracellularly throughout the whole cytoplasm, including lysosomes and Golgi apparatus. In vitro cell experiments showed that SICN nanoparticles were more toxic than single components, and HGC-27 cells had more absorption and higher toxicity to nanoparticles under slightly acidic conditions. Conclusion: SICN is a promising carrier-free nanoparticle, and the combination of two single-component therapies can exert a synergistic antitumor effect. When exposed to a tumor acidic environment, SICN showed stronger cytotoxicity due to charge conversion. More importantly, the nanoparticles&apos; self-monitoring function has been developed, opening up new ideas for combined tumor therapy.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

21001 - Nano-materials (production and properties)

Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Nanomedicine

ISSN

1178-2013

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

Neuveden

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

2487-2499

UT code for WoS article

000634522200001

EID of the result in the Scopus database

2-s2.0-85103829362