Marine Oomycetes of the Genus Halophytophthora Harbor Viruses Related to Bunyaviruses

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F20%3A43918186" target="_blank" >RIV/62156489:43410/20:43918186 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.3389/fmicb.2020.01467" target="_blank" >https://doi.org/10.3389/fmicb.2020.01467</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2020.01467" target="_blank" >10.3389/fmicb.2020.01467</a>

Result language

angličtina

Original language name

Marine Oomycetes of the Genus Halophytophthora Harbor Viruses Related to Bunyaviruses

Original language description

We investigated the incidence of RNA viruses in a collection of Halophytophthora spp. from estuarine ecosystems in southern Portugal. The first approach to detect the presence of viruses was based on the occurrence of dsRNA, typically considered as a viral molecule in plants and fungi. Two dsRNA-banding patterns (TILDE OPERATOR+D917 and 9 kb) were observed in seven of 73 Halophytophthora isolates tested (9.6%). Consequently, two dsRNA-hosting isolates were chosen to perform stranded RNA sequencing for de novo virus sequence assembly. A total of eight putative novel virus species with genomic affinities to members of the order Bunyavirales were detected and their full-length RdRp gene characterized by RACE. Based on the direct partial amplification of their RdRp gene by RT-PCR multiple viral infections occur in both isolates selected. Likewise, the screening of those viruses in the whole collection of Halophytophthora isolates showed that their occurrence is limited to one single Halophytophthora species. To our knowledge, this is the first report demonstrating the presence of negative (MINUS SIGN ) ssRNA viruses in marine oomycetes.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/EF15_003%2F0000453" target="_blank" >EF15_003/0000453: Phytophthora Research Centre</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

15 July

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

1467

UT code for WoS article

000556883300001

EID of the result in the Scopus database

2-s2.0-85088795863