The demonstration of the GFLV Nepovirus isolates on naturally infected grapevine cultivars and evaluation of variability within genome region encoding movement protein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F11%3A00170456" target="_blank" >RIV/62156489:43510/11:00170456 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

The demonstration of the GFLV Nepovirus isolates on naturally infected grapevine cultivars and evaluation of variability within genome region encoding movement protein

Original language description

In this work, the infection of Grapevine fanleaf virus (GFLV) disease was described on the base of symptomatic differences within eight grapevine plants of six grape ciltivars with positive tests on GFLV. Among them, cultivars Kodrjanka, Pamjati Negrula,Kišmiš Lučistyj were planted in wine region of the South Moravia (Czech Republic), three infected grapevine cultivars (URS, Cinsaut, Dimrit) included in this study originating from Italy. Except symptomatic evaluation, the differences between isolates were emphasized at the genetic level too, exactly in the frame of RNA2 genomic region coding movement protein. The variability of the tested isolates within the eight plants was in the range from 86.59 to 97.61% at the nucleotide level. The results confirmed very high degree of similarity between virus isolates of GFLV within studied RNA2 region. This fact was assessed by the phylogenetic analysis of obtained sequencing data too.

Czech name

The demonstration of the GFLV Nepovirus isolates on naturally infected grapevine cultivars and evaluation of variability within genome region encoding movement protein

Czech description

In this work, the infection of Grapevine fanleaf virus (GFLV) disease was described on the base of symptomatic differences within eight grapevine plants of six grape ciltivars with positive tests on GFLV. Among them, cultivars Kodrjanka, Pamjati Negrula,Kišmiš Lučistyj were planted in wine region of the South Moravia (Czech Republic), three infected grapevine cultivars (URS, Cinsaut, Dimrit) included in this study originating from Italy. Except symptomatic evaluation, the differences between isolates were emphasized at the genetic level too, exactly in the frame of RNA2 genomic region coding movement protein. The variability of the tested isolates within the eight plants was in the range from 86.59 to 97.61% at the nucleotide level. The results confirmed very high degree of similarity between virus isolates of GFLV within studied RNA2 region. This fact was assessed by the phylogenetic analysis of obtained sequencing data too.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/QH91214" target="_blank" >QH91214: New biotechnolgical approaches for nepovirus resitance creation in grapevine rootstock cultivars.</a><br>

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

ISSN

1211-8516

e-ISSN

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Volume of the periodical

59

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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