Genetic variability of Grapevine fanleaf virus isolates within genes 1B(HEL) and 1E(POL)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F11%3A00174486" target="_blank" >RIV/62156489:43510/11:00174486 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Genetic variability of Grapevine fanleaf virus isolates within genes 1B(HEL) and 1E(POL)
Original language description
The genetic variability of six isolates of Grapevine fanleaf virus (GFLV) from South Moravia (Czech Republic) and Italy was investigated by RT-PCR and sequencing within part of the RNA-1-encoded genes 1B(Hel) (379 nts) and 1E(Pol) (365 nts). Sequence analyses showed 96-100% and 91.4-100% identity at the amino acid level for genes 1B(Hel) and 1E(Pol), respectively, among Czech, Italian and other isolates for which sequence information is available. As expected, some of the conserved motifs of the helicase and polymerase proteins were found in the deduced amino acid sequences, although isolates PN33 from Czech Republic, and isolates 55TK and UR11 from Italy had a Ile to Val subtitution in the helicase motif B. Phylogenetic analyses confirmed the close relationship among GFLV isolates and the relatedness of GFLV isolate WAPN173 with Arabis mosaic virus (ArMV) in protein 1B(Hel). Two distinct phylogenetic clusters were identified for protein 1E(Pol), with isolates from California and the F
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/QH91214" target="_blank" >QH91214: New biotechnolgical approaches for nepovirus resitance creation in grapevine rootstock cultivars.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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