Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F14%3A00223083" target="_blank" >RIV/62156489:43510/14:00223083 - isvavai.cz</a>
Result on the web
<a href="http://scialert.net/abstract/?doi=ijv.2014.243.252" target="_blank" >http://scialert.net/abstract/?doi=ijv.2014.243.252</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3923/ijv.2014.243.252" target="_blank" >10.3923/ijv.2014.243.252</a>

Result language
angličtina
Original language name
Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein
Original language description
In this study, we reported sequencing and molecular analysis in detail of central part of 2BMP gene (approximately 270 nts) in case of Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). For more complex analysis of genetic variability we added reference sequences of evaluated nepoviruses and also sequence of phylogenetically very similar Nepovirus GDefV. Range of sequence analysis showed 74.5-99.3% identity at the nucleotide level and 86.5-100% identity at the amino acid level for partial gene 2BMP among Czech, Italian, German, Swiss sequences and references of all isolates. In this study eleven isolates were sequenced consisting of 5 GFLV and 6 ArMV isolates. As expected, GFLV and ArMV sequences were distinguished based on sequence identities but differences were not significant. Surprisingly, GDefV sequence was almost identical with the majority of GFLV sequences, even more than some GFLV sequences between each other. Local complexity of nucleotide sequences was also eval
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GE - Plant cultivation
OECD FORD branch
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Project
<a href="/en/project/QH91214" target="_blank" >QH91214: New biotechnolgical approaches for nepovirus resitance creation in grapevine rootstock cultivars.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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