Virus elimination in peach using chemotherapy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F17%3A43913124" target="_blank" >RIV/62156489:43510/17:43913124 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1155.64" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2017.1155.64</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1155.64" target="_blank" >10.17660/ActaHortic.2017.1155.64</a>

Result language
angličtina
Original language name
Virus elimination in peach using chemotherapy
Original language description
Chemotherapy is one of the methods used for the elimination of viruses in plants, based on the use of chemicals with antiviral effect. The most commonly used antiviral is ribavirin. In this study the effect of acyclovir and rimantadine on peach viruses was examined. The virus diseases PDV and PNRSV were detected in the peach cultivar 'Suncrest' and PPV in the peach cultivar 'Redhaven'. A nodal culture was established (following surface disinfection with mercuric chloride) and the young shoots were multiplied. The antivirals in the total amounts of 25 or 50 mg L-1 were applied into the MS medium using a sterile filter, just after autoclavage. Shoots were cultivated on this type of medium for three weeks and transferred on Quoirin and Lepoivre medium containing plant growth regulators and without antivirals. After two months of cultivation, the shoots were tested using the reverse transcription PCR and the products were separated electrophoretically on 1.5% agarose gel. The study showed that the usage of acyclovir is more effective than rimantadine.
Czech name
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Czech description
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Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project
<a href="/en/project/QJ1210175" target="_blank" >QJ1210175: Research and development of standard methodic procedures of recovering of fruit trees and grapevinesby chemotherapy of in vitro cultures for certification system of healthy status of planting material.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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