INITIATION AND MAINTENANCE OF CUCUMBER (Cucumis sativus L.) EMBRYOGENIC CELL SUSPENSION

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F01%3A21100007" target="_blank" >RIV/62156489:_____/01:21100007 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
INITIATION AND MAINTENANCE OF CUCUMBER (Cucumis sativus L.) EMBRYOGENIC CELL SUSPENSION
Original language description
Embryogenic cell suspension cultures from hypocotyl segments of cucumber (Cucumis sativus L.) have been initiated by 7 days exposure to Murashige and Skoog medium supplemented with 3% sucrose, 2.2 mg l-1 2,4-dichlorophenoxyacetic acid, and 100 mg l-1 casein hydrolyzate. The suspension cultures were maintained in the same medium with 1.1 mg l-1 2,4- dichlorophenoxyacetic acid only on a horizontal shaker at 28°C, and 16/8 h photoperiod, subcultivated every week. The cell suspension cultures contained heterogeneous arrays of several different types of single cells (size < 200 ?m) and cell clusters (size ? 200 ?m). The average generation time was 3 days. The somatic embryos developed after transfer from auxin-containing medium to auxin-free medium. Embryogenic capacity of the cell lines varied between 35 and 470 somatic embryos per 1 ml of cell suspension.
Czech name
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Czech description
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Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
17th International Conference on Plant Growth Substances
ISBN
80-7157-518-6
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
118-118
Publisher name
Neuveden
Place of publication
Brno, Czech Republic
Event location
Brno, Czech Republic
Event date
Jul 1, 2001
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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