Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F09%3A00137108" target="_blank" >RIV/62156489:43210/09:00137108 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Long-term suspension cultures of cucumber (Cucumis sativus L.) with high embryogenic potential
Original language description
Cucumber (Cucumis sativus L.) cytokinin-independent embryogenic cell suspension cultures were derived and maintained for more than three and half years without losing the embryogenic potential. The preparation and the characteristics of the cucumber embryogenic cell suspension possess many similarities to that of carrot. The cultures were induced from hypocotyl explants of in vitro grown cucumber plants in liquid MS media containing 2,4-dichlorophenoxyacetic acid as the sole growth regulator during sixweeks and they contained a heterogeneous array of several different types of single cells and cell clusters (PEMs). The established cell suspensions were subcultured in one-week intervals, while the inoculation density was optimized to 2.0 x 105 cells ml-1 using cell viability as a marker. Somatic embryos were obtained after the transfer of the proembryogenic masses to a hormone-free semisolid MS medium with a frequency of 388 plus-minus 57 somatic embryos per 1 ml of packed cell volume
Czech name
Dlouhodobé suspenzní kultury okurky (Cucumis sativus L.) s vysokým embryogenním potenciálem
Czech description
Cytokinin independentní embryogenní buněčná suspenze okurky (Cucumis sativus L.) byla odvozena a udržována více než tři a půl roku bez ztráty embryogenního potenciálu.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Physiologiae Plantarum
ISSN
0137-5881
e-ISSN
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Volume of the periodical
DOI 10.100
Issue of the periodical within the volume
00902774
Country of publishing house
PL - POLAND
Number of pages
7
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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