Effect of pre-treatment and division of Norway spruce zygotic embryo on uptake of 14C-2,4-D and induction of somatic embryogenesis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F01%3A21100015" target="_blank" >RIV/62156489:_____/01:21100015 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Effect of pre-treatment and division of Norway spruce zygotic embryo on uptake of 14C-2,4-D and induction of somatic embryogenesis
Original language description
The main task of this work was to study reiations between uptake of 2,4-dichiorophenoxy acetic acid (2,4-D), an important tooi of sornatic embryogenesis induction, zygotic embryo pre-treatment and injury, that were found as increasing the subsequent induction of embryogenic cultures. The seeds were pre-treated in dark at 4 C under sterile distilied water for 0, 3, 5 and 7 days. The uptake of 14C-2, 4-D was significantly higher after 7 days treatment. The way of injury (division) ofzygotic embryos also modified uptake of 14C-2, 4-D to zygotic embryo and its parts. Results showed that the fastest shift of 14C-2,4-D into radicles (both intact and separated) was aligned with nearly none embryogenic cultures induction. On the contrary the Iowest uptake of 2,4-D into cotyledonary part was aligned with the highest number of achieved cultures. Hypocotyls showed intermediate results in both parameters.
Czech name
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Czech description
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Project
<a href="/en/project/GV522%2F96%2FK186" target="_blank" >GV522/96/K186: Preservation and reproduction of gene sources of main species of coniferous and broadleaved forest trees</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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