Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F02%3A21100001" target="_blank" >RIV/62156489:_____/02:21100001 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers

Original language description

Random amplified polymorphic DNA (RAPD) markers were used for the determination of the genetic stability and relationship of clones of Norway spruce (Picea abies (L.) Karst.) early somatic embryo cultures. RAPD analysis of the genetic relationship was carried out on 16 clones of somatic embryos (14 clones derived in our laboratory and two control clones derived in foreign laboratories). Twenty pre-selected primers produced 88 reproducible polymorphic and monomorphic bands, which were evaluated using theRAPDistance Program 1.04. The genetic stability during in vitro culture was studied on 6 clones derived in our laboratory (Brno, Velká Klajdovka) and two control clones. The genomic DNA was isolated in regular intervals 5 times a year during 4 subsequent years. No genetic instability was determined during the 4-year period using 4 selected primers with the highest polymorphisms.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GC - Plant growing, crop rotation

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia

ISSN

0006-3088

e-ISSN

—

Volume of the periodical

57

Issue of the periodical within the volume

4

Country of publishing house

SK - SLOVAKIA

Number of pages

5

Pages from-to

517-521

UT code for WoS article

—

EID of the result in the Scopus database

—