Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F03%3A21100018" target="_blank" >RIV/62156489:_____/03:21100018 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker
Original language description
In 1997 and 1998 we used samples of harvested grain to verify the possibility of distinguishing 14 winter wheat genotypes and six triticale genotypes and detecting the impurity on the basis of the detection of polymorphism of prolamin kernel proteins using the methods of the PAGE ISTA. On the basis of the identity index two sister prolamin Iines with different percentage of participation, which was based on the weather conditions of the year of harvest, were discovered in seven wheat genotypes (Astella,Brea, Hana, Ilona, Siria, Sofia and Šárka) and two triticale genotypes (Tomádo and KM 779). A foreign genotype was detected in the Hana and Astella varieties. The identity index of the impurity to the Astella and Hana variety (i.e. ii = 0. 28 and ii = 0.20, respectively) was considerably Iower. In an unknown genotype (impurity) we detected the gliadin block Gld lB3, which is the genetic marker of rye translocation TIBL.IRS, the Sr31 gene of resistance to black rust.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GC - Plant growing, crop rotation
OECD FORD branch
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Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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