Development of Multilocus Sequence Tool for typing Cryptosporidium muris and Cryptosporidium andersoni
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F11%3A43870237" target="_blank" >RIV/62157124:16170/11:43870237 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1128/JCM.01329-10" target="_blank" >http://dx.doi.org/10.1128/JCM.01329-10</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/JCM.01329-10" target="_blank" >10.1128/JCM.01329-10</a>
Alternative languages
Result language
angličtina
Original language name
Development of Multilocus Sequence Tool for typing Cryptosporidium muris and Cryptosporidium andersoni
Original language description
Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, two of the most common gastric Cryptosporidium spp. infecting mammals. In this study,we screened the C. muris whole-genome sequencing data for microsatellite and minisatellite sequences. Among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were eventually chosen. DNA sequence analyses of 27 C. muris and 17 C. andersoni DNA preparations showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all four loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isola
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Result continuities
Project
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Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of clinical microbiology
ISSN
0095-1137
e-ISSN
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Volume of the periodical
49
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
34-41
UT code for WoS article
000285787100003
EID of the result in the Scopus database
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