Preliminary epitope mapping of Torque teno sus virus 1 and 2 putative capsid protein and serological detection of infection in pigs

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F13%3A43872222" target="_blank" >RIV/62157124:16170/13:43872222 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/13:43872222
Result on the web
<a href="http://dx.doi.org/10.1099/vir.0.050500-0" target="_blank" >http://dx.doi.org/10.1099/vir.0.050500-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1099/vir.0.050500-0" target="_blank" >10.1099/vir.0.050500-0</a>

Result language
angličtina
Original language name
Preliminary epitope mapping of Torque teno sus virus 1 and 2 putative capsid protein and serological detection of infection in pigs
Original language description
The aim of this work is to identify antigenic regions within the ORF1 protein of Torque teno sus virus 1 (TTSuV1) and Torque teno virus sus 2 (TTSuV2) that could be used as antigens to detect virus-specific antibodies following infection in pigs. Proteinsequences of TTSuV ORF1 genes were analysed to predict linear antigenic epitopes. Synthesized peptides were analysed for serological reactivity with swine sera. Such an antigenic region was identified at the C terminus of the ORF1 protein of both viruses and showed serological reactivity with 78 % (TTSuV1) and 88 % (TTSuV2) of swine sera. An ELISA with an immunodominant peptide as antigen was used to examine the sera of piglets, aged 4-20 weeks, and adults. Results indicated that TTSuV1- and TTSuV2-specific antibodies were detectable at 4 weeks. Antibody titres increased from week 10 and peaked at week 20. A relatively high antibody titre persisted to adulthood.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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