c-Myb contributes to endochondral bone development

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F14%3A43873326" target="_blank" >RIV/62157124:16170/14:43873326 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
c-Myb contributes to endochondral bone development
Original language description
The expression of c-Myb was analyzed in fore- and hindlimbs of mouse embryos during early embryonic stages using in situ hybridization. c-MYB protein was detected throughout prenatal development using immunohistochemistry. In the limb, weak expression ofc-myb was observed in embryos at E12.5. At later stages (E13.5 and E14.5), expression was located in the perichondrium and interdigital areas, which correlated with localization of c-MYB protein. Furthermore, we detected the c-MYB protein in pre-hypertrophic and hypertrophic chondrocytes. Transient overexpression of c-myb by transfection of a c-Myb-coding vector markedly increased the formation of cartilage nodules and the production of extracellular matrix, as detected by intense staining with AlcianBlue. Moreover, the expression of Sox9, a major marker of chondrogenesis, was increased. A loss-of-function approach using c-myb specific siRNA decreased nodule formation, as well as downregulating the level of Sox9 expression. Furthermor
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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