The comparison of the methods for the identification of pathogenic serotypes and biotypes of Yersinia enterocolitica: Microbiological methods and PCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F06%3A00000874" target="_blank" >RIV/62157124:16270/06:00000874 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The comparison of the methods for the identification of pathogenic serotypes and biotypes of Yersinia enterocolitica: Microbiological methods and PCR

Original language description

In this study, pathogenic strains of Y. enterocolitica were identified by microbiological and PCR methods. The samples were collected from pigs, cattle, poultry, and slaughter houses. Three common techniques were used to isolate Y. enterocolitica from the samples - ITC, PSB, and direct on the CIN. Primers A1/A2, Y1/Y2, and rfbC 1/rfbC 2 were used for the specific detection of the pathogenic strains of Y. enterocolitica. Traditional microbiological methods were found to be insufficient for the specific identification of the Y. enterocolitica pathogen. In comparison with PCR which was able to detect 149 strains, the biochemical test could detect only 138 species. These results show that the use of biochemical methods of cultivation did not allow the identification of all Y. enterocolitica pathogens. In total, 149 strains of pathogenic Y. enterocolitica were examined of which 120 were from pigs, 19 from poultry, 8 were cattle strains, and 2 came from the environments of slaughterhouses.

Czech name

Porovnání metod pro identifikaci patogenních sérotypů a biotypů Yersinia enterocolitica : Mikrobiologické metody a PCR

Czech description

Studie byla zaměřena na identifikaci patogenních kmenů Y. enterocolitica mikrobiologickými, biochemickými a PCR metodami. Vzorky byly odebírány z prasat, skotu, drůbeže a prostředí porážek. Pro izolaci Y. enterocolitica ze vzorků byly použity mikrobiologické metody s využitím kultivačních půd ITC, PSB, CIN. Patogenní kmeny Y. enterocolitica byly detekovány biochemickými a PCR metodami. Biochemickými metodami bylo identifikováno 138 patogenních kmenů Y. enterocolitica a PCR metodami 149 patogenních kmenů. PCR metody použité pro identifikaci patogenních kmenů jsou tedy více specifická než metody biochemické. Z celkového počtu odebraných vzorků (4617) bylo izolováno 149 patogenních kmenů Y. enterocolitica (120 kmenů z prasat, 19 z drůbeže, 8 ze skotu a 2kmeny z prostředí porážek).

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Czech Journal of Food Science

ISSN

1212-1800

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

217-222

UT code for WoS article

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EID of the result in the Scopus database

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