The rapid determination of bromadiolone in liver and blood plasma by in-injector pyrolysis gas chromatography-Ion trap tandem mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F17%3A43875945" target="_blank" >RIV/62157124:16270/17:43875945 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jchromb.2017.10.027" target="_blank" >http://dx.doi.org/10.1016/j.jchromb.2017.10.027</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jchromb.2017.10.027" target="_blank" >10.1016/j.jchromb.2017.10.027</a>
Alternative languages
Result language
angličtina
Original language name
The rapid determination of bromadiolone in liver and blood plasma by in-injector pyrolysis gas chromatography-Ion trap tandem mass spectrometry
Original language description
The unintentional poisoning of off-target animals by bromadiolone, a second generation anticoagulant rodenticide, is an undesirable outcome requiring sensitive analytical methods. In this study, a rapid and sensitive method for the determination of bromadiolone in liver and blood plasma by means of gas chromatography coupled with tandem mass spectrometry without need for derivatization was developed. The method is based on the in-injector pyrolysis of bromadiolone and subsequent gas chromatography coupled with ion trap tandem mass spectrometry with electron ionization. Sample preparation includes extraction with methanol, evaporation under nitrogen stream, and dissolution in toluene. The pyrolysis of bromadiolone was carried out in an injector at 390 °C. Chromatographic separation of the pyrolytical fragment of bromadiolone was achieved using a VF?5 ms column with helium as the mobile phase. Tandem in-time mass spectrometry of the separated pyrolytical fragment of bromadiolone was carried out using an ion trap mass spectrometer after electron ionization. Recovery ranged from 94 to 98%. The method showed good linearity up to 1000 ?g kg?1 for liver and 1000 ?g L?1 for plasma. The limit of detection was 0.38 ?g kg?1 for liver and 0.26 ?g L?1 for plasma. The developed method was used successfully in several animal poisoning cases.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography B
ISSN
1570-0232
e-ISSN
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Volume of the periodical
1070
Issue of the periodical within the volume
DEC 2017
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
4
Pages from-to
117-120
UT code for WoS article
000417661900017
EID of the result in the Scopus database
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