PCR-RFLP identification of meat from red deer, sika deer, roe deer, fallow deer, mouflon, wild boar, hare and cattle
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877351" target="_blank" >RIV/62157124:16270/19:43877351 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/19:43877351
Result on the web
<a href="https://actavet.vfu.cz/media/pdf/actavet_2019088010103.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2019088010103.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb201988010103" target="_blank" >10.2754/avb201988010103</a>
Alternative languages
Result language
angličtina
Original language name
PCR-RFLP identification of meat from red deer, sika deer, roe deer, fallow deer, mouflon, wild boar, hare and cattle
Original language description
Meat authentication is currently a key topic in relation to the quality and safety of food of animal origin at all levels of production and the global distribution chain. New polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) based on digestion of PCR products with two restriction enzymes, MboII and AciI, have been developed for the specific identification of raw and heat-processed meat from red deer (Cervus elaphus), sika deer (Cervus nippon), roe deer (Capreolus capreolus), fallow deer (Dama dama), mouflon (Ovis musimon), wild boar (Sus scrofa), hare (Lepus europaeus) and cattle (Bos taurus). The PCR primers were targeted in a well-conserved region of the cytochrome b (CYTB) gene to amplify a 378 bp region of all the analysed species. This simple, rapid and cost-effective method is suitable for identification of the meat of game species and their possible substitution by beef.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta veterinaria Brno
ISSN
0001-7213
e-ISSN
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Volume of the periodical
88
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
9
Pages from-to
103-111
UT code for WoS article
000468126600013
EID of the result in the Scopus database
2-s2.0-85065507358