A quantitative comparison of two kits for DNA extraction from canned tuna
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877381" target="_blank" >RIV/62157124:16270/19:43877381 - isvavai.cz</a>
Alternative codes found
RIV/00027162:_____/19:N0000090
Result on the web
<a href="https://actavet.vfu.cz/media/pdf/actavet_2019088030315.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2019088030315.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb201988030315" target="_blank" >10.2754/avb201988030315</a>
Alternative languages
Result language
angličtina
Original language name
A quantitative comparison of two kits for DNA extraction from canned tuna
Original language description
The most common methods that can be used for species identification of tuna include methods based on detection of species-specific DNA via the polymerase chain reaction (PCR) method. The problem with DNA detection in processed products is the possibility of DNA fragmentation during the technological process. The quantity and quality of extracted DNA is a crucial step for species identification based on the DNA analysis. In this study, two DNA extraction methods (DNeasy Blood & Tissue Kit and DNeasy mericon Food Kit) for tuna DNA isolation were compared. Eight food products of canned tuna (three of them were declared as Thunnus albacares and five products were declared as Katsuwonus pelamis) with a different addition of various ingredients were tested. Furthermore, three different times of proteolysis (30 min, 60 min, overnight) for each sample and each extraction kit were evaluated. The DNA concentration was determined by a Qubit dsDNA HS Assay Kit fluorescence method and quantified using a Qubit fluorometer. The DNA purity was evaluated using the A260/A280 ratio of absorbances measured on a spectrophotometer. The main indicator of DNA quality and quantity was its amplifiability in the subsequent real-time PCR for Thunnus species, Thunnus albacares and Katsuwonus pelamis. Based on the results, both kits can be used for tuna species determination in highly heat-treated products with different composition, nevertheless, the DNeasy mericon Food Kit provided better statistical values in some parameters. The effect of different times of proteolysis was significant in most of the samples with regard to the crossing point values determined by real-time PCR.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/QJ1530272" target="_blank" >QJ1530272: Complex strategies for effective detection of food fraud in the chain production-consumer</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta veterinaria Brno
ISSN
0001-7213
e-ISSN
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Volume of the periodical
88
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
8
Pages from-to
315-322
UT code for WoS article
000509757500009
EID of the result in the Scopus database
2-s2.0-85073443810