First Report of plasmid mediated colistin resistance from wildlife in Russia

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43878237" target="_blank" >RIV/62157124:16270/19:43878237 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/19:43878237
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
First Report of plasmid mediated colistin resistance from wildlife in Russia
Original language description
The aim of this study was to select Enterobacteriaceae isolates with plasmid-mediated colistin resistance genes from cloacal swabs of Eurasian Black and Red Kites. Isolates (13.8%, n=168) that showed reduced susceptibility to colistin (3.5mg/L) were identified using MALDI-TOF MS and tested for mobile colistin resistance encoded by mcr genes using PCR. One mcr-1-positive E. coli isolate was detected and selected for further testing. The isolate was resistant to colistin (MIC > 4mg/L) and susceptible to 18 antibiotics by broth dilution and disk diffusion methods, respectively. WGS using MiSeq (Illumina) revealed the mcr-1-positive E. coli sample belonged to ST2280. The mcr-1 gene was located on conjugative IncI2 plasmid pDR164 (59891 bp, GenBank number MK542639). Plasmid pDR164 showed similarity to other mcr-1-carrying IncI2 plasmids found in E. coli isolates from swine, poultry, human and sewage in USA, Vietnam, Argentina and China. Comparison of pDR164 with other plasmids revealed high similarity in the plasmid backbone and the region carrying mcr. In this study, we report the first mcr-1-positive isolate in wildlife in Russia
Czech name
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Czech description
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Project
<a href="/en/project/NV18-09-00605" target="_blank" >NV18-09-00605: Enterobacteriaceae resistant to carbapenems and colistin: One-Health approach in addressing human and non-human sources of emerging antimicrobial resistance mechanisms</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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