Direct ELLA: qualitative method for insects determination

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F23%3A43880758" target="_blank" >RIV/62157124:16270/23:43880758 - isvavai.cz</a>
Result on the web
<a href="https://www.vetuni.cz/files/upload/733/KMVP%202023_Sbornik.pdf" target="_blank" >https://www.vetuni.cz/files/upload/733/KMVP%202023_Sbornik.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Direct ELLA: qualitative method for insects determination
Original language description
The polysaccharide chitin and its subunits are found in large quantities in insects and participate in the construction of the entire exoskeleton. One of the ways to verify the presence of insects in food and thereby ensure their safety is to detect this polysaccharide, more precisely N-acetyl-D-glucosamine. Only lectin has the ability to bind specific polysaccharides, therefore this work was focused on optimizing the ELLA (enzyme-linked-lectin assay) method. Chitosan and an extract of the house cricket (Acheta Domesticus) were used as a model sample. The limit of detection (LODλ= 0.3532) was determined for this method. Chitosan samples at concentrations of 0.01 and 0.005 g/ml and cricket extract samples were confirmed as positive by the ELLA method.
Czech name
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Czech description
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Project
<a href="/en/project/QK23020101" target="_blank" >QK23020101: The comprehensive laboratory strategy for identification of insect species intended for human consumption and the production of processed animal protein, authentication of insect-based foods.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
XXV. konference mladých vědeckých pracovníků s mezinárodní účastí: Sborník příspěvků
ISBN
978-80-7305-928-6
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
18-21
Publisher name
Veterinární univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
May 24, 2023
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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