Development of Indirect Sandwich ELLA for Detection of Insects in Food

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F24%3A43881406" target="_blank" >RIV/62157124:16270/24:43881406 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/24:98681 RIV/60461373:22330/24:43930885

Result on the web

<a href="https://www.mdpi.com/2076-3417/14/23/10794/pdf?version=1732195718" target="_blank" >https://www.mdpi.com/2076-3417/14/23/10794/pdf?version=1732195718</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/app142310794" target="_blank" >10.3390/app142310794</a>

Result language

angličtina

Original language name

Development of Indirect Sandwich ELLA for Detection of Insects in Food

Original language description

Edible insects have been officially classified as food in the European Union since 2015. Currently, four insect species are approved for food use. However, no official method for detecting insects in food has been recognized to date. To establish a simple analytical method for insect detection in food, we developed an indirect sandwich (enzyme-linked lectin sorbent assay) ELLA specific for N-acetylglucosamine in chitin and chitosan polymers. The validation of the method demonstrated that the ELLA developed in this study is reliable for insect detection. The limit of detection (LOD) and quantification (LOQ) were 0.006 and 0.028 mg/mL, respectively. Intra-day precision ranged from 2.45% to 30.29%, and inter-day precision from 0.36% to 12.87%. Significant differences in the total amount of chitin and chitosan were observed among the insect products, processed insect products, and samples without any insect addition (p &lt; 0.05).

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

21101 - Food and beverages

Project

<a href="/en/project/QK23020101" target="_blank" >QK23020101: The comprehensive laboratory strategy for identification of insect species intended for human consumption and the production of processed animal protein, authentication of insect-based foods.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Sciences-Basel

ISSN

2076-3417

e-ISSN

2076-3417

Volume of the periodical

14

Issue of the periodical within the volume

23

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

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UT code for WoS article

001376873800001

EID of the result in the Scopus database

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