Optimizing Insect Analysis with Raman Spectroscopy: Assessing Polar Solvents Suitability and pH Impact

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F24%3A43881462" target="_blank" >RIV/62157124:16270/24:43881462 - isvavai.cz</a>
Result on the web
<a href="https://fvhe.vetuni.cz/files/upload/733/KMVP%202024_Sbornik.pdf" target="_blank" >https://fvhe.vetuni.cz/files/upload/733/KMVP%202024_Sbornik.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Optimizing Insect Analysis with Raman Spectroscopy: Assessing Polar Solvents Suitability and pH Impact
Original language description
The main objective of this study was to investigate the suitability of polar solvents and their pH influence on extracting selected edible insects for their detection by Raman spectroscopy. Dried specimens of Acheta domesticus (house cricket), Zophobas morio L. (superworm), and Tenebrio molitor L. (mealworm) were analysed. Extraction utilized four polar solvents at specified pH levels: distilled water (pH 7.0), PBS (Phosphate Buffered Saline; pH 2.4, 7.4, 9.4), TBS (Tris Buffered Saline; pH 7.4), and Citrate Buffer (pH 6), in a 1:5 solvent-to-sample ratio. Raman spectroscopy verified the discernibility of solvents from insect samples, thus validating their appropriateness for detection purposes. Through the recording of Raman spectra of detected insects and subsequent statistical analysis, it was determined that PBS at pH 9.4 was the most suitable solvent, characterized by the highest signal attenuation and optimal peak resolution, even when applied to closely related insect species.
Czech name
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Czech description
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Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
XXVI. konference mladých vědeckých pracovníků s mezinárodní účastí: Sborník příspěvků
ISBN
978-80-7305-957-6
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
8-10
Publisher name
Veterinární univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
Jun 5, 2024
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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