Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F25%3A43882797" target="_blank" >RIV/62157124:16270/25:43882797 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/25:00143603

Result on the web

<a href="https://actavet.vfu.cz/media/pdf/actavet_2025094040317.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2025094040317.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202594040317" target="_blank" >10.2754/avb202594040317</a>

Result language

angličtina

Original language name

Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues

Original language description

The aim of this study was to monitor the growth of Bacillus cereus in 11 different types of naturally contaminated ready-to-cook plant-based meat analogues (PBMAs). The PBMAs were prepared according to the manufacturer’s instructions and subsequently stored at room temperature for up to 24 h. Bacillus spp. represented the second most numerous group of microbiota detected in PBMAs after lactic acid bacteria. Bacillus cereus sensu lato (s.l.) was isolated from all 11 PBMA samples. Following heat treatment and 24-h storage at 22 °C, B. cereus s.l. was detected by plate counting in five samples, with one product reaching nearly 4 log CFU/g. The presence of B. cereus sensu stricto (s.s.) was detected in all 11 samples. Bacillus thuringiensis was present in 7 samples, and in 4 PBMA samples the species B. weihenstephanensis, B. mycoides, and B. cytotoxicus were found. A total of 95.7% of B. cereus s.l. strains contained genes for toxin production. The presence of nhe genes was demonstrated in 92.5%, hbl genes in 59.0% of the analysed B. cereus s.l. strains, the cytK-1 gene in 5 strains (B. cytotoxicus). The presence of the ces gene was confirmed in 5% of B. cereus s.l. strains. In total, 47 different combinations of genes for toxin production were detected in B. cereus s.l. strains. The almost 100% presence of virulence genes associated with the possibility of B. cereus growth during inappropriate storage of PBMAs thus poses a risk for the development of foodborne diseases.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

<a href="/en/project/EH23_015%2F0008175" target="_blank" >EH23_015/0008175: Innovation of Czech Infrastructure for Integrative Structural Biology</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

1801-7576

Volume of the periodical

94

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

317-327

UT code for WoS article

001651916000007

EID of the result in the Scopus database

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