Determination of antioxidant activity using oxidative damage to plasmid DNA: pursuit of solvent optimization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F13%3A43871809" target="_blank" >RIV/62157124:16370/13:43871809 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.2478/s11696-013-0334-8" target="_blank" >http://dx.doi.org/10.2478/s11696-013-0334-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11696-013-0334-8" target="_blank" >10.2478/s11696-013-0334-8</a>

Result language

angličtina

Original language name

Determination of antioxidant activity using oxidative damage to plasmid DNA: pursuit of solvent optimization

Original language description

Oxidative stress plays a key role in the pathophysiology of many diseases. Hydroxyl radical is the oxidative species most commonly causing damage to cells. The aim of this work was to optimize the method for antioxidant activity determination on a modellipophilic geranylated flavanone, diplacone. This method uses protection of plasmid DNA from oxidation by a hydroxyl radical generated by the Fenton reaction involving oxidation of metal ions using H2O2 and ascorbate. The method was optimized for lipophilic compounds using several solvents and co-solvents. It was found that (2-hydroxypropyl)-beta-cyclodextrin (0.1 mass % aq. sol.) is the best co-solvent for our model lipophilic compound to measure the antioxidant activity by the method presented. Othersolvents, namely dimethyl sulfoxide, Cremophor ELA (R) (0.1 mass % aq. sol.), ethanol, and methanol, were not suitable for the determination of the antioxidant activity by the method described. Tween 80 (0.1 mass % aq. sol.) and a mixture

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FR - Pharmacology and apothecary chemistry

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Papers - Chemické Zvesti

ISSN

0366-6352

e-ISSN

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Volume of the periodical

67

Issue of the periodical within the volume

5

Country of publishing house

SK - SLOVAKIA

Number of pages

6

Pages from-to

484-489

UT code for WoS article

000314882900002

EID of the result in the Scopus database

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