Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F14%3A43872716" target="_blank" >RIV/62157124:16370/14:43872716 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.carres.2014.03.010" target="_blank" >http://dx.doi.org/10.1016/j.carres.2014.03.010</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.carres.2014.03.010" target="_blank" >10.1016/j.carres.2014.03.010</a>

Result language
angličtina
Original language name
Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography
Original language description
A fast liquid chromatography method for separation and determination of myo-inositol is reported. Determination of the biologically important isomer of inositols, myo-inositol, was optimized to avoid overlapping to possible interferents according to European Pharmacopoeia (glycerol, D-mannitol) and saccharose. The method in HILIC mode is extremely selective to other carbohydrates which allows to separate myo-inositol from allo-and D-chiro-inositol with resolution 12.3 and 5.2, resp. and this way it enables to separate myo-inostiol from contingent carbohydrates present in a sample matrix. Retention time of myo-inositol was 12 min at 10 degrees C, though higher temperatures (25 degrees C or 40 degrees C) or higher water content in the mobile phase couldspeed up the separation and determination to four minutes. LOD of the method was 9 mg/L at 10 degrees C, and 5 mg/L at 25 degrees C, resp.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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