Activity of Na /K -activated Mg2 -dependent ATP-hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp Rod-9

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F15%3A43873469" target="_blank" >RIV/62157124:16370/15:43873469 - isvavai.cz</a>

Result on the web

<a href="http://actavet.vfu.cz/84/1/0003/" target="_blank" >http://actavet.vfu.cz/84/1/0003/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201585010003" target="_blank" >10.2754/avb201585010003</a>

Result language

angličtina

Original language name

Activity of Na /K -activated Mg2 -dependent ATP-hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp Rod-9

Original language description

The aim of our work was to study Na+/K+-activated Mg2+-dependent ATPase activity in cellfree extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 isolated from the human large intestine, and to carry out the kinetic analysis of the enzyme reaction. The maximum ATPase activity for both bacterial strains at +35 oC was determined. The highest activities of the studied enzyme in the cell-free extracts of D. piger Vib-7 at pH 7.0 and Desulfomicrobium sp. Rod-9 at pH 6.5 were measured. Based on experimental data, the analysis of kinetic properties of the ATP-hydrolase reaction by the studied bacteria was carried out. The enzyme activity, initial (instantaneous) reaction rate (V0) and maximum rate of the ATPase reaction (Vmax) was significantly higher in D. piger Vib-7 cells than in Desulfomicrobium sp. Rod-9. Michaelis constants (Km) of the enzyme reaction for both bacterial strains were determined.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

<a href="/en/project/EE2.3.30.0053" target="_blank" >EE2.3.30.0053: Pharmaco-toxicological evaluation of newly synthesized (isolated) compounds as an integration tool for pre-clinical disciplines at VFU Brno</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

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Volume of the periodical

84

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

3-12

UT code for WoS article

000351657800001

EID of the result in the Scopus database

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