Genome-wide single nucleotide variation in Toxoplasma gondii type II isolates from Europe

Result code in IS VaVaI

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Alternative codes found

RIV/62157124:16170/21:43879374

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Genome-wide single nucleotide variation in Toxoplasma gondii type II isolates from Europe

Original language description

Introduction. Toxoplasma gondii is a highly prevalent protozoan parasite infecting a very broad range of animals and humans worldwide. A better knowledge of the genetic diversity and population structure of T. gondii may help to understand transmission routes and sources of infection for livestock and humans.There is limited data on genome-wide comparisons of field isolates re presenting the same clonal lineage. The aim of the present study was therefore to assess genome-wide genetic diversity among T. gondii clonal lineage II isolates from Europe, where this lineage is predominant. Methods To this end, four type II isolates obtained from Germany, the Czech Republic, Greece and France were used and DNA samples of these isolates were subjected to whole genome sequencing using the Illumina NextSeq platform. Results. For each of the T. gondii isolates, at least 95 % of the reads were mapped to reference genomes. The mapped reads covered over 99 % of the type II reference genome (ME49, ToxoDB, v47) with a read depth of &gt;20 per base. In total, 10185, 10462 and 11003 Single Nucleotide Variations (SNVs) were detected in isolates from Germany, the Czech Republic and Greece. The lowest number of SNVs (n = 4212) was observed in the isolate from France. The highest number of unique/isolate-specific SNVs was found in an isolate from Germany (n = 2249) followed by the Czech (n = 2165) and Greece (n = 1921) type II isolates. The type II strain from France revealed the lowest number of unique SNVs (n = 765). Conclusion. This pilot study demonstrated considerable genetic variation among four European clonal type II isolates and helped to provide new insights into the population structure of T. gondii parasites.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Parasiten: alte und neue Herausforderungen

ISBN

978-3-86345-577-4

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

71-72

Publisher name

DVG Service GmbH

Place of publication

Leipzig

Event location

Giesen

Event date

Jun 28, 2021

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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