Characterization of the Penetration of the Blood-Brain Barrier by High-Performance Liquid Chromatography (HPLC) Using a Stationary Phase with an Immobilized Artificial Membrane
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F18%3A50014450" target="_blank" >RIV/62690094:18470/18:50014450 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/18:43889557 RIV/00216208:11410/18:10376622 RIV/00179906:_____/18:10376622
Result on the web
<a href="http://dx.doi.org/10.1080/00032719.2018.1424175" target="_blank" >http://dx.doi.org/10.1080/00032719.2018.1424175</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/00032719.2018.1424175" target="_blank" >10.1080/00032719.2018.1424175</a>
Alternative languages
Result language
angličtina
Original language name
Characterization of the Penetration of the Blood-Brain Barrier by High-Performance Liquid Chromatography (HPLC) Using a Stationary Phase with an Immobilized Artificial Membrane
Original language description
The biological activity of drugs on organisms is associated with the pharmacokinetic properties, such as the ability to penetrate through environments of varying polarity such as cellular organelles. In this area, particular attention is turned to the physicochemical properties that determine the potential of drugs to pass across the blood-brain barrier and thus to act on the central nervous system. In this study, special effort has been devoted to the simulation of passive diffusion of seven drugs (propranolol, ibuprofen, atenolol, promazine, chlorpromazine, imipramine, and desipramine) through the blood-brain barrier by high-performance liquid chromatography (HPLC) using a column with an immobilized artificial membrane. Gradient reverse elution was used to develop a linear correlation model for the capacity factors k(IAM) and the in vivo logarithmic values of brain-to-blood drug concentration ratios (log BB) with R of 0.9851. Eleven additional pharmaceuticals were determined by the same method to predict their potential to penetrate the blood-brain barrier. The reported analytical method represents an alternative tool for rapid and noninvasive assessment of the absorption properties of chemicals, especially for the development of novel drugs. The retention of the studied compounds on the immobilized artificial membrane column was also compared with three other C18-based stationary phases. Herein, the results of the HPLC determination of drugs using an immobilized artificial membrane are briefly discussed with respect to a general application of the method for evaluating a broader spectrum of pharmaceutical compounds.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical letters
ISSN
0003-2719
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
15
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
2401-2414
UT code for WoS article
000436859100007
EID of the result in the Scopus database
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