Tracking minor clones in chronic lymphocytic leukemia using high-throughput immunoprofiling

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F18%3A00068760" target="_blank" >RIV/65269705:_____/18:00068760 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/18:00103335
Result on the web
<a href="http://phdretreat.ceitec.cz/ceitec-phd-and-postdoc-retreat-2018/" target="_blank" >http://phdretreat.ceitec.cz/ceitec-phd-and-postdoc-retreat-2018/</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Tracking minor clones in chronic lymphocytic leukemia using high-throughput immunoprofiling
Original language description
In chronic lymphocytic leukemia (CLL), multiple clones can be detected in up to a quar-ter of cases. Typically, a major clone coexists with minor one(s). Shifts in clonal ratios and prevailing of initially minor clones in the course of the disease have been reported, therefore, their monitoring is desirable. We aimed to test the capability of NGS im-munoprofiling methods to detect minor clones in oligoclonal CLL. We focused primar-ily on cases showing result discrepancies using various methods, and cases with con-siderable clonal shifts during disease course. Overall, 21 samples from 14 patients (7 patients were monitored in several time-points) were submitted to NGS immunoprofiling with DNA and RNA as a starting material. The results from both methods were compared to those obtained using bulk sample Sanger sequencing and single cell analysis. We report that detection of minor clones in oligoclonal CLL is challenging, since its efficiency varies with different approaches. As the clinic-biological significance of minor clones in general is still unclear, it warrants further detailed investigation in a larger cohort.
Czech name
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Czech description
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Project
<a href="/en/project/NV15-30015A" target="_blank" >NV15-30015A: Analysis of clonal heterogeneity in chronic lymphocytic leukemia using next generation sequencing of B cell receptor. A national study.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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