Detection and Functional Analysis of TP53 Mutations in CLL

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00070639" target="_blank" >RIV/65269705:_____/19:00070639 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/protocol/10.1007%2F978-1-4939-8876-1_6" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-4939-8876-1_6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-8876-1_6" target="_blank" >10.1007/978-1-4939-8876-1_6</a>

Result language
angličtina
Original language name
Detection and Functional Analysis of TP53 Mutations in CLL
Original language description
Chronic lymphocytic leukemia (CLL) represents a prototype disease in which TP53 gene defects lead to inferior prognosis. Here, we present two distinct methodologies which can be used to identify TP53 mutations in CLL patients; both protocols are primarily intended for research purposes. The functional analysis of separated alleles in yeast (FASAY) can be flexibly adapted to a variable number of samples and provides an immediate functional readout of identified mutations. Amplicon-based next-generation sequencing then allows for a high throughput and accurately detects subclonal TP53 variants (sensitivity<1% of mutated cells).
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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