Immunomagnetic separation of Escherichia coli O26, O111 and O157 from vegetables.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F01%3A22013036" target="_blank" >RIV/67179843:_____/01:22013036 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Immunomagnetic separation of Escherichia coli O26, O111 and O157 from vegetables.
Original language description
Raw vegetable and fruit products have been increasingly connected with human infections with Shiga toxin-producing Escherichia coli. This study evaluated the isolation and detection of E. coli O26, O111 and O157 inoculated into vegetable samples using immunomagnetic particles. Standard cultivation and immunomagnetic separation (IMS) procedures were compared. It was found that immunomagnetic particles very efficiently concentrate E. coli cells, detecting significantly more bacteria then with standard cultivation procedures. E. coli O111and O157 were detected in 93-100 % of inoculated samples using IMS, but only 36-53 % samples after plating were found positive. In the case of E. coli O26, 93 % samples after direct plating were positive compared with 100% positive samples after IMS; the number of presumptive colonies was significantly higher after IMS.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
<a href="/en/project/GA203%2F98%2F1145" target="_blank" >GA203/98/1145: Development of methods for isolation of biologically active compounds using magnetic separation techniques</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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