Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F03%3A20030011" target="_blank" >RIV/67985823:_____/03:20030011 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport.

Original language description

Mutants were constructed for mitochondrial uncoupling protein UCP1, with single or multiple substitutions within or nearby the UCP-signatures located in the 1st alfa-helix and 2nd matrix segment, using the QuickChange site directed mutagenesis protocol (Stratagene), and were assayed fluorometrically for kinetics of fatty acid (FA)-induced H+ uniport and for Cl- uniport. Their ability to bind 3H-GTP was also evaluated. The wild type UCP1 was associated with the FA-induced H+ uniport proportional to the added protein with a Km for lauric acid of 43 mikroM and Vmax of 18 mikromol.min-1.(mg protein)-1. Neutralization of Arg 152 (in the 2nd matrix segment UCP-signature) led to ~50% reduction of FA affinity (reciprocal Km) and of Vmax for FA-induced H+ uniport. Halved FA-affinity and 70% reduction of Vmax was found for the double His substitution outside the signature (H145L & H147L mutant). Neutralization of Asp 27 in the1st alfa-helix UCP-signature (D27V mutant) resulted in 75% reduction o

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Biochemistry & Cell Biology

ISSN

1357-2725

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

212-220

UT code for WoS article

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EID of the result in the Scopus database

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