Distribution of mitochondrial nucleoids upon mitochondrial network fragmentation and network reintegration in HEPG2 cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F13%3A00391723" target="_blank" >RIV/67985823:_____/13:00391723 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.biocel.2012.11.019" target="_blank" >http://dx.doi.org/10.1016/j.biocel.2012.11.019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.biocel.2012.11.019" target="_blank" >10.1016/j.biocel.2012.11.019</a>
Alternative languages
Result language
angličtina
Original language name
Distribution of mitochondrial nucleoids upon mitochondrial network fragmentation and network reintegration in HEPG2 cells
Original language description
Mitochondrial DNA (mtDNA) is organized in nucleoids in complex with accessory proteins, proteins of mtDNA replication and gene expression machinery. A robust mtDNA genome is represented by hundreds to thousands of nucleoids in cell mitochondrion. We usedconfocal microscopy to study mitochondrial nucleoid redistribution upon mitochondrial fission and following reintegration of the mitochondrial network. Fission was induced by oxidative stress at respiration inhibition by rotenone or upon elimination ofthe protonmotive force by uncoupling or upon canceling its electrical component, delta psi(m), by valinomycin; and by silencing of mitofusin MFN2. Agent withdrawal resulted in concomitant mitochondrial network reintegration. We found two major principalmorphological states: (i) a tubular state of the mitochondrial network with equidistant nucleoid spacing, and (ii) a fragmented state of solitary spheroid objects in which several nucleoids were clustered. Reintegration of fragments into
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Biochemistry and Cell Biology
ISSN
1357-2725
e-ISSN
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Volume of the periodical
45
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
593-603
UT code for WoS article
000315539900011
EID of the result in the Scopus database
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