Sex difference in the sensitivity of cardiac mitochondrial permeability transition pore to calcium load

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00458439" target="_blank" >RIV/67985823:_____/16:00458439 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s11010-015-2619-4" target="_blank" >http://dx.doi.org/10.1007/s11010-015-2619-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11010-015-2619-4" target="_blank" >10.1007/s11010-015-2619-4</a>

Result language

angličtina

Original language name

Sex difference in the sensitivity of cardiac mitochondrial permeability transition pore to calcium load

Original language description

Most of the experimental studies have revealed that female heart is more tolerant to ischemia/reperfusion (I/R) injury as compared with the male myocardium. It is widely accepted that mitochondrial dysfunction, and particularly mitochondrial permeability transition pore (MPTP) opening, plays a major role in determining the extent of cardiac I/R injury. The aim of the present study was, therefore, to analyze (i) whether calcium-induced swelling of cardiac mitochondria is sex-dependent and related to the degree of cardiac tolerance to I/R injury and (ii) whether changes in MPTP components—cyclophilin D (CypD) and ATP synthase—can be involved in this process. We have observed that in mitochondria isolated from rat male and female hearts the MPTP has different sensitivity to the calcium load. Female mitochondria are more resistant both in the extent and in the rate of the mitochondrial swelling at higher calcium concentration (200 µM). At low calcium concentration (50 µM) no differences were observed. Our data further suggest that sex-dependent specificity of the MPTP is not the result of different amounts of ATP synthase and CypD, or their respective ratio in mitochondria isolated from male and female hearts. Our results indicate that male and female rat hearts contain comparable content of MPTP and its regulatory protein CypD; parallel immunodetection revealed also the same contents of adenine nucleotide translocator or voltage-dependent anion channel. Increased resistance of female heart mitochondria thus cannot be explained by changes in putative components of MPTP, and rather reflects regulation of MPTP function.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FA - Cardiovascular diseases including cardio-surgery

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular and Cellular Biochemistry

ISSN

0300-8177

e-ISSN

—

Volume of the periodical

412

Issue of the periodical within the volume

1-2

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

147-154

UT code for WoS article

000369943600016

EID of the result in the Scopus database

2-s2.0-84958874206