Major role of adipocyte prostaglandin E2 in lipolysis-induced macrophage recruitment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00459242" target="_blank" >RIV/67985823:_____/16:00459242 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1194/jlr.M066530" target="_blank" >http://dx.doi.org/10.1194/jlr.M066530</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1194/jlr.M066530" target="_blank" >10.1194/jlr.M066530</a>

Result language

angličtina

Original language name

Major role of adipocyte prostaglandin E2 in lipolysis-induced macrophage recruitment

Original language description

Obesity induces accumulation of adipose tissue macrophages (ATMs), which contribute to both local and systemic inflammation and modulate insulin sensitivity. Adipocyte lipolysis during fasting and weight loss also leads to ATM accumulation, but without proinflammatory activation suggesting distinct mechanisms of ATM recruitment. We examined the possibility that specific lipid mediators with anti-inflammatory properties are released from adipocytes undergoing lipolysis to induce macrophage migration. In the present study, we showed that conditioned medium (CM) from adipocytes treated with forskolin to stimulate lipolysis can induce migration of RAW 264.7 macrophages. In addition to FFAs, lipolytic stimulation increased release of prostaglandin E2 (PGE2) and prostaglandin D2 (PGD2), reflecting cytosolic phospholipase A2 α activation and enhanced cyclooxygenase (COX) 2 expression. Reconstituted medium with the anti-inflammatory PGE2 potently induced macrophage migration while different FFAs and PGD2 had modest effects. The ability of CM to induce macrophage migration was abolished by treating adipocytes with the COX2 inhibitor sc236 or by treating macrophages with the prostaglandin E receptor 4 antagonist AH23848. In fasted mice, macrophage accumulation in adipose tissue coincided with increases of PGE2 levels and COX1 expression. Collectively, our data show that adipocyte-originated PGE2 with inflammation suppressive properties plays a significant role in mediating ATM accumulation during lipolysis.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FB - Endocrinology, diabetology, metabolism, nutrition

OECD FORD branch

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Project

<a href="/en/project/LH14040" target="_blank" >LH14040: Modulation of Fatty Acid Reesterification in Adipose Tissue by Lipid Mediators</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Lipid Research

ISSN

0022-2275

e-ISSN

—

Volume of the periodical

57

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

663-673

UT code for WoS article

000373924600015

EID of the result in the Scopus database

2-s2.0-84963864745