Counting NMDA Receptors at the Cell Surface
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00459534" target="_blank" >RIV/67985823:_____/16:00459534 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-4939-2812-5_3" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-2812-5_3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-2812-5_3" target="_blank" >10.1007/978-1-4939-2812-5_3</a>
Alternative languages
Result language
angličtina
Original language name
Counting NMDA Receptors at the Cell Surface
Original language description
In the mammalian central nervous system, N-methyl-d-aspartate (NMDA) receptors mediate fast excitatory synaptic transmission. These receptors also play a key role in brain development, synaptic plasticity, and memory formation, and their dysregulation can cause neurological and/or psychiatric disorders. A variety of techniques have been applied to examine the molecular mechanisms that regulate the number of NMDA receptors at the cell surface. Here we provide a step-by-step guides for two commonly used techniques: a quantitative assay that measures both surface and total receptor pools, and a biotinylation assay that can rapidly evaluate the surface and total expression levels of receptor subunits. Among other applications, these methods can be used to measure the cell surface expression of recombinant receptors with genetic alterations and the effect of co-expressing additional proteins that may regulate subunit surface trafficking. These techniques can be used either individually or in parallel experiments, thus allowing researchers to independently validate observations regarding the regulation of NMDA receptor expression.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
FH - Neurology, neuro-surgery, nuero-sciences
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA14-02219S" target="_blank" >GA14-02219S: Regulation of NMDA receptor trafficking and function by N-glycosylation</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Ionotropic Glutamate Receptor Technologies
ISBN
978-1-4939-2811-8
Number of pages of the result
13
Pages from-to
31-44
Number of pages of the book
304
Publisher name
Humana Press Inc.
Place of publication
Totowa
UT code for WoS chapter
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