Evaluation of the potential of chitosan/beta-1,3-glucan/hydroxyapatite material as a scaffold for living bone graft production in vitro by comparison of ADSC and BMDSC behaviour on its surface

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00474278" target="_blank" >RIV/67985823:_____/17:00474278 - isvavai.cz</a>

Alternative codes found

RIV/00064211:_____/17:W0000030

Result on the web

<a href="http://dx.doi.org/10.1088/1748-605X/aa56f9" target="_blank" >http://dx.doi.org/10.1088/1748-605X/aa56f9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1748-605X/aa56f9" target="_blank" >10.1088/1748-605X/aa56f9</a>

Result language

angličtina

Original language name

Evaluation of the potential of chitosan/beta-1,3-glucan/hydroxyapatite material as a scaffold for living bone graft production in vitro by comparison of ADSC and BMDSC behaviour on its surface

Original language description

The opinion regarding the origin of adult stem cells that should be used for living bone construct generation is strongly divided in the scientific community. In this study, the potential of chitosan/beta-1,3glucan/hydroxyapatite (chit/glu/HA) material as a scaffold for bone regeneration applications was evaluated by behaviour comparison of adult stem cells derived from both origins-adipose derived mesenchymal stem cell (ADSC) tissue and bone marrow derived mesenchymal stem cells (BMDSCs). In the case of ADSC isolation, low and high negative pressures were applied during a liposuction procedure in order to determine if negative pressure settings may have an impact on subsequent cell behaviour in vitro. The obtained results demonstrated that the chit/glu/HA material is a promising candidate to be used for living bone graft production in vitro as both ADSCs and BMDSCs revealed a satisfactory proliferation and differentiation ability on its surface. Nevertheless, BMDSCs would be a better choice of adult stem cells since they were better spread, more strongly attached and showed a more superior proliferation and differentiation ability than ADSCs when cultured on the chit/glu/HA scaffold. However, if BMDSCs cannot be isolated, ADSCs may be used for bone construct production but lipoaspirate should be collected under low negative pressure (-200mm Hg), as high negative pressure (-700mm Hg) applied during liposuction surgery may retard subsequent ADSC proliferation and type I collagen production.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Project

<a href="/en/project/NV15-33018A" target="_blank" >NV15-33018A: Application of adipose tissue-derived stem cells obtained by liposuction in tissue engineering</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Materials

ISSN

1748-6041

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

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UT code for WoS article

000395985600005

EID of the result in the Scopus database

2-s2.0-85016015656