Cytoprotective activity of mitochondrial uncoupling protein‐2 in lung and spleen

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F18%3A00489819" target="_blank" >RIV/67985823:_____/18:00489819 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/2211-5463.12410" target="_blank" >http://dx.doi.org/10.1002/2211-5463.12410</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/2211-5463.12410" target="_blank" >10.1002/2211-5463.12410</a>

Result language

angličtina

Original language name

Cytoprotective activity of mitochondrial uncoupling protein‐2 in lung and spleen

Original language description

Mitochondrial uncoupling protein-2 (UCP2) mediates free fatty acid (FA)-dependent H+ translocation across the inner mitochondrial membrane (IMM), which leads to acceleration of respiration and suppression of mitochondrial superoxide formation. Redox-activated mitochondrial phospholipase A2 (mt-iPLA2 gamma) cleaves FAs from the IMM and has been shown to acts in synergy with UCP2. Here, we tested the mechanism of mt-iPLA2 gamma-dependent UCP2-mediated antioxidant protection using lipopolysaccharide (LPS)-induced pro-inflammatory and pro-oxidative responses and their acute influence on the overall oxidative stress reflected by protein carbonylation in murine lung and spleen mitochondria and tissue homogenates. We provided challenges either by blocking the mt-iPLA2 gamma function by the selective inhibitor R-bromoenol lactone (R-BEL) or by removing UCP2 by genetic ablation. We found that the basal levels of protein carbonyls in lung and spleen tissues and isolated mitochondria were higher in UCP2-knockout mice relative to the wild-type (wt) controls. The administration of R-BEL increased protein carbonyl levels in wt but not in UCP2-knockout (UCP2-KO) mice. LPS further increased the protein carbonyl levels in UCP2-KO mice, which correlated with protein carbonyl levels determined in wt mice treated with R-BEL. These results are consistent with the UCP2/mt-iPLA2 gamma antioxidant mechanisms in these tissues and support the existence of UCP2-synergic mt-iPLA2 gamma-dependent cytoprotective mechanism in vivo.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA15-02051S" target="_blank" >GA15-02051S: The role of redox-sensitive mitochondrial phospholipase iPLA2γ in the regulation of cellular antioxidant protection</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEBS Open Bio

ISSN

2211-5463

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

692-701

UT code for WoS article

000428997200019

EID of the result in the Scopus database

2-s2.0-85043451803