Trabecular Architecture Determines Impulse Propagation Through the Early Embryonic Mouse Heart

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00503876" target="_blank" >RIV/67985823:_____/19:00503876 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/19:10396072

Result on the web

<a href="https://doi.org/10.3389/fphys.2018.01876" target="_blank" >https://doi.org/10.3389/fphys.2018.01876</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphys.2018.01876" target="_blank" >10.3389/fphys.2018.01876</a>

Result language

angličtina

Original language name

Trabecular Architecture Determines Impulse Propagation Through the Early Embryonic Mouse Heart

Original language description

Most embryonic ventricular cardiomyocytes are quite uniform, in contrast to the adult heart, where the specialized ventricular conduction system is molecularly and functionally distinct from the working myocardium. We thus hypothesized that the preferential conduction pathway within the embryonic ventricle could be dictated by trabecular geometry. Mouse embryonic hearts of the Nkx2.5:eGFP strain between ED9.5 and ED14.5 were cleared and imaged whole mount by confocal microscopy, and reconstructed in 3D at 3.4 mu m isotropic voxel size. The local orientation of the trabeculae, responsible for the anisotropic spreading of the signal, was characterized using spatially homogenized tensors (3 x 3 matrices) calculated from the trabecular skeleton. Activation maps were simulated assuming constant speed of spreading along the trabeculae. The results were compared with experimentally obtained epicardial activation maps generated by optical mapping with a voltage-sensitive dye. Simulated impulse propagation starting from the top of interventricular septum revealed the first epicardial breakthrough at the interventricular grove, similar to experimentally obtained activation maps. Likewise, ectopic activation from the left ventricular base perpendicular to dominant trabecular orientation resulted in isotropic and slower impulse spreading on the ventricular surface in both simulated and experimental conditions. We conclude that in the embryonic pre-septation heart, the geometry of the A-V connections and trabecular network is sufficient to explain impulse propagation and ventricular activation patterns.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30106 - Anatomy and morphology (plant science to be 1.6)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in physiology

ISSN

1664-042X

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

Jan 8

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

1876

UT code for WoS article

000455141200001

EID of the result in the Scopus database

2-s2.0-85065483200