Reversible and Irreversible Modulation of Tubulin Self-Assembly by Intense Nanosecond Pulsed Electric Fields
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00507886" target="_blank" >RIV/67985823:_____/19:00507886 - isvavai.cz</a>
Alternative codes found
RIV/67985882:_____/19:00507886 RIV/68378050:_____/19:00507886
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/adma.201903636" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/adma.201903636</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/adma.201903636" target="_blank" >10.1002/adma.201903636</a>
Alternative languages
Result language
angličtina
Original language name
Reversible and Irreversible Modulation of Tubulin Self-Assembly by Intense Nanosecond Pulsed Electric Fields
Original language description
Tubulin self-assembly into microtubules is a fascinating natural phenomenon. Its importance is not just crucial for functional and structural biological processes, but it also serves as an inspiration for synthetic nanomaterial innovations. The modulation of the tubulin self-assembly process without introducing additional chemical inhibitors/promoters or stabilizers has remained an elusive process. This work reports a versatile and vigorous strategy for controlling tubulin self-assembly by nanosecond electropulses (nsEPs). The polymerization assessed by turbidimetry is dependent on nsEPs dosage. The kinetics of microtubules formation is tightly linked to the nsEPs effects on structural properties of tubulin, and tubulin-solvent interface, assessed by autofluorescence, and the zeta potential. Moreover, the overall size of tubulin assessed by dynamic light scattering is affected as well. Additionally, atomic force microscopy imaging reveals the formation of different assemblies reflecting applied nsEPs. It is suggested that changes in C-terminal modification states alter tubulin polymerization-competent conformations. Although the assembled tubulin preserve their integral structure, they might exhibit a broad range of new properties important for their functions. Thus, these transient conformation changes of tubulin and their collective properties can result in new applications
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Advanced Materials
ISSN
0935-9648
e-ISSN
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Volume of the periodical
31
Issue of the periodical within the volume
39
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
e1903636
UT code for WoS article
000481222200001
EID of the result in the Scopus database
2-s2.0-85070672049