Selection of optimal reference genes for gene expression studies in chronically hypoxic rat heart
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00510047" target="_blank" >RIV/67985823:_____/19:00510047 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs11010-019-03584-x" target="_blank" >https://link.springer.com/article/10.1007%2Fs11010-019-03584-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11010-019-03584-x" target="_blank" >10.1007/s11010-019-03584-x</a>
Alternative languages
Result language
angličtina
Original language name
Selection of optimal reference genes for gene expression studies in chronically hypoxic rat heart
Original language description
Adaptation to chronic hypoxia renders the heart more tolerant to ischemia/reperfusion injury. To evaluate changes in gene expression after adaptation to chronic hypoxia by RT-qPCR, it is essential to select suitable reference genes. In a chronically hypoxic rat model, no specific reference genes have been identified in the myocardium. This study aimed to select the best reference genes in the left (LV) and right (RV) ventricles of chronically hypoxic and normoxic rats. Sprague-Dawley rats were adapted to continuous normobaric hypoxia (CNH, 12% O-2 or 10% O-2) for 3 weeks. The expression levels of candidate genes were assessed by RT-qPCR. The stability of genes was evaluated by NormFinder, geNorm and BestKeeper algorithms. The best five reference genes in the LV were Top1, Nupl2, Rplp1, Ywhaz, Hprt1 for the milder CNH and Top1, Ywhaz, Sdha, Nupl2, Tomm22 for the stronger CNH. In the RV, the top five genes were Hprt1, Nupl2, Gapdh, Top1, Rplp1 for the milder CNH and Tomm22, Gapdh, Hprt1, Nupl2, Top1 for the stronger CNH. This study provides validation of reference genes in LV and RV of CNH rats and shows that suitable reference genes differ in the two ventricles and depend on experimental protocol.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular and Cellular Biochemistry
ISSN
0300-8177
e-ISSN
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Volume of the periodical
461
Issue of the periodical within the volume
1-2
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
15-22
UT code for WoS article
000490391400002
EID of the result in the Scopus database
2-s2.0-85068909319