Kappa but not delta or mu opioid receptors form homodimers at low membrane densities
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00549802" target="_blank" >RIV/67985823:_____/21:00549802 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/21:10441796
Result on the web
<a href="https://doi.org/10.1007/s00018-021-03963-y" target="_blank" >https://doi.org/10.1007/s00018-021-03963-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00018-021-03963-y" target="_blank" >10.1007/s00018-021-03963-y</a>
Alternative languages
Result language
angličtina
Original language name
Kappa but not delta or mu opioid receptors form homodimers at low membrane densities
Original language description
Opioid receptors (ORs) have been observed as homo- and heterodimers, but it is unclear if the dimers are stable under physiological conditions, and whether monomers or dimers comprise the predominant fraction in a cell. Here, we use three live-cell imaging approaches to assess dimerization of ORs at expression levels that are 10-100 x smaller than in classical biochemical assays. At membrane densities around 25/mu m(2), a split-GFP assay reveals that kappa OR dimerizes, while mu OR and delta OR stay monomeric. At receptor densities < 5/mu m(2), single-molecule imaging showed no kappa OR dimers, supporting the concept that dimer formation depends on receptor membrane density. To directly observe the transition from monomers to dimers, we used a single-molecule assay to assess membrane protein interactions at densities up to 100 x higher than conventional single-molecule imaging. We observe that kappa OR is monomeric at densities < 10/mu m(2) and forms dimers at densities that are considered physiological. In contrast, mu OR and delta OR stay monomeric even at the highest densities covered by our approach. The observation of long-lasting co-localization of red and green kappa OR spots suggests that it is a specific effect based on OR dimerization and not an artefact of coincidental encounters.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA17-05903S" target="_blank" >GA17-05903S: Alterations of δ-opioid receptor mobility and function by acute and chronic depletion of plasma membrane cholesterol content of live cells</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cellular and Molecular Life Sciences
ISSN
1420-682X
e-ISSN
1420-9071
Volume of the periodical
78
Issue of the periodical within the volume
23
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
7557-7568
UT code for WoS article
000707846600001
EID of the result in the Scopus database
2-s2.0-85117590664